- What is the function of degasser in HPLC?
- How can HPLC be used to determine purity?
- What is assay percentage?
- Why assay is more than 100?
- What assay means?
- What is assay value?
- What is assay test in HPLC?
- What can HPLC detect?
- What is peak purity in HPLC?
- What is difference between potency and purity?
- Why assay is performed?
- How is potency calculated?
What is the function of degasser in HPLC?
The Vacuum Degasser is designed to remove gases from a liquid such as the mobile phase used in HPLC..
How can HPLC be used to determine purity?
What does it tell us? High Performance Liquid Chromatography (HPLC) We use this method to determine the purity of our products. The ratio of the desired product to that of the combined impurities is expressed as a % purity. We will typically state that a product is say >99% (by HPLC).
What is assay percentage?
The verb assay means to “examine a chemical to determine its purity.” In our context, assay provides a metric that refers to the chemicals purity, usually expressed as a percent assay. On occasion, assay refers to the purity of the chemical, e.g., 99% or 99.9% pure.
Why assay is more than 100?
HPLC assay higher than 100% If your values are much higher than 100%, such as 110%, then the reason very likely is that your standard and your samples are not the same. In other words the purity of your standard is less than 100%, or lower than stated in the certificate of analysis.
What assay means?
1 : examination and determination as to characteristics (such as weight, measure, or quality) 2 : analysis (as of an ore or drug) to determine the presence, absence, or quantity of one or more components also : a test used in this analysis. 3 : a substance to be assayed also : the tabulated result of assaying.
What is assay value?
i. The quantity of an ore’s valuable constituents, determined by multiplying its assay grade or percentage of valuable constituents by its dimensions. The figure for precious metals is generally given in troy ounces per ton of ore, or per assay ton. See Also: assay grade, value.
What is assay test in HPLC?
Services HPLC Testing & UPLC Testing. … HPLC stands for High Performance Liquid Chromatography, and is a technique used to separate different constituents of a compound using high pressure to push solvents through the column. It is the most widely used technique to identify, quantify and separate components of a mixture.
What can HPLC detect?
High-performance liquid chromatography or high-pressure liquid chromatography (HPLC) is a chromatographic method that is used to separate a mixture of compounds in analytical chemistry and biochemistry so as to identify, quantify or purify the individual components of the mixture.
What is peak purity in HPLC?
Peak purity is a comparison of the reference standard to the API in the sample stressed by ‘forced degradation (thus specificity). In essence you are showing that no impurity (related substance) is eluting underneath the main API peak in HPLC.
What is difference between potency and purity?
A quick definition would be: -Potency is a measure of drug activity expressed in terms of the amount required to produce an effect of given intensity. -Purity is a measure of the amount of API present in a sample compared to those of related substances, impurities, residual solvents, etc.
Why assay is performed?
An assay is an investigative (analytic) procedure in laboratory medicine, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity (the analyte).
How is potency calculated?
The formula provided by CLSI to calculate the potency is shown as: Potency = (Assay purity) * (Active fraction) * (1-Water Content).